Porcine DNA Detection (Halal Test)

Halal-based industries especially in food sector have been expanding rapidly in the global market. Not only in Muslim countries, developing non-Muslim countries are now giving priority to Halal-Certified products due to quality and safety aspects. Thus, Halal testing is no longer solely a religious concern.

Testing and certification of Halal products is improving due to the growing demands and role. There are various detection method of pork contamination available in the market. Rapid immunoassay tests are convenient and inexpensive, however the trade-off is the sensitivity and accuracy. Culture plates are accurate and reliable but also require longer process time. Thus, PCR and qPCR is by far the best option in terms of specificity, sensitivity and speed.

Principle:

PKL PorcineTrace PCR kit enables detection of porcine DNA down to 5pg of porcine DNA traces in food products within approximately 3-4 hours (excluding DNA extraction process) to result analysis. The test is based on conventional PCR triplex plateform which amplifies a porcine specific gene together with an external amplification control and a universal vertebrate gene as internal control. The test has been optimized to favour porcine DNA amplification. This test has been validated for specificity to porcine DNA.

Product insert available here:

In line with the above product, we have also introduced the PKL Porcine qPCR Detection kit which utilizes a real time approach and fluorescence-based PCR method. We employed the dual-labeled probe method of detection, i.e. Taqman(R) probe in the kit which increases its specificity.